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IMMUNOFLUORESCENCE PATTERNS
Various IF patterns can be asked in exams.hereby attaching the same
1.Best test to detect ANA is by INDIRECT IMMUNOFLUORESCENCE METHOD.It uses Hep2 cells
2.For detection of P ANCA and C ANCA only ELISA is necessary.No need of ANA profiles

Image 2

Detection of antibodies against dsDNA.

Indication: systemic lupus erythematosus

Initial dilution: 1:10, conjugate class anti-human IgG, FITC-labelled.

Animal-pathogenic haemoflagellates of Crithidia luciliae are used for the detection of autoantibodies against double-stranded, native DNA (dsDNA, nDNA) with indirect immunofluorescence. These protozoa possess a giant mitochondrion containing dsDNA (kinetoplast) that, in general, does not show any of the other antigens present in the cell nuclei. Antibodies reacting with the kinetoplast are therefore only directed against dsDNA

Image 3 ANCA patterns

Kindly try the AIIMS AND JIPMER test series for similar questions